| Article DOI |
10.1039/c2cp42738a |
| Data DOI |
N/A |
| Internal ID |
10.1039/c2cp42738a/1 |
| Membrane composition (molar fraction) |
| POPC |
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine |
1 |
| PAPC |
1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine |
1 |
|
| Solution composition |
Pure water
|
| Temperature (K) |
300 |
| Total hydration |
50 (mass %) |
| pH |
UNKNOWN |
| Reagent sources |
| POPC |
d31-POPC and POPC, Avanti Polar Lipids, Alabaster, AL, USA |
| H2O |
UNKNOWN |
|
| Sample protocol |
Lipid samples were prepared by dissolving in organic solvent followed by
solvent evaporation and vacuum drying. Samples were hydrated to form
multilamellar vesicles under controlled conditions. Then samples were centrifuged
and supernatants were discarded. Precise total hydration is unknown.
Paper cites for the closest protocol: 10.1529/biophysj.107.112847
|
| NMR method |
PDLF:R18_1^7 |
| NMR instrument |
Bruker Avance AVII-500 NMR with 4 mm CP-MAS HX probe |
| NMR RF heating correction |
heating was checked using methanol-filled rotor |
| NMR sign measured |
NONE |
| NMR details |
The following setup was used: spectral width of 149.5 ppm;
refocused INEPT with t1 and t2 of 1.79 ms and 1.19 ms respectively;
CP contact time equal to 700 ms; radiofrequency pulses set to give the nutation
frequencies: 45.00 kHz (R1871 pulses), 63.45 kHz (13C 90-
and 180-pulses), 63.45 kHz (1H INEPT pulses), 50 kHz
(1H decoupling pulses), 50.76–63.45 kHz (1H CP ramp pulse
during contact time), 53.66 kHz (13C CP pulse during contact
time); t1 with increments equal to 399.6 ms (0% colesterol, 7%
and 15% cholesterol) and 132.2 ms (34% colesterol, 50% and
60% cholesterol); for each t1 value, 256 scans were recorded
during an acquisition time of 100 ms using 5 s recycle
delay. MAS frequency 5kHz was used.
|
| Show on GitHub |
experiments/OrderParameters/10.1039/c2cp42738a/1 main |
| Name |
Value |
| PH_METHOD |
UNKNOWN
|
Order Parameters POPC
View JSON main
No OP Data Available for PAPC