Experiment OP - 10.1021/bi991573v/1

Article DOI 10.1021/bi991573v
Data DOI N/A
Internal ID 10.1021/bi991573v/1
Membrane composition (molar fraction)
PAPC 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine 1
Solution composition Pure water
Temperature (K) 310
Total hydration 95 (mass %)
pH 7.4
Reagent sources
PAPC Avanti (Birmingam, AL)
H2O Deuterium-depleted water
Sample protocol NMR samples consisted of 22 mg of 31d-PAPC. Lipids were mixed as CHCl3 solutions and then dried under N2 and vacuum. MLV suspensions (28−60 mM) were prepared by vigorous vortexing of lipids in 0.6 mL of bufer solution.
NMR method 2H:QE
NMR instrument Custom-built spectrometer (Davis, 1979) operating at 46.2 MHz
NMR RF heating correction UNKNOWN
NMR sign measured NONE
NMR details The typical spectrum resulted from 24−48k scans of the two-pulse sequence with a 90° pulse of 3.75−3.95 μs, an interpulse spacing of 40 μs, and a dwell time of 2 μs. The delay between acquisitions was 300 ms, and 4K data points per acquisition were collected in quadrature with 8-cyclops phase cycling. Spectra were de-Paked.
Show on GitHub experiments/OrderParameters/10.1021/bi991573v/1 main

Name Value
ADDITIONAL_MOLECULES
Key Value
TRIS tris(hydroxymethyl)aminomethane, 20 mM
EDTA Ethylenediaminetetraacetic acid, 0.5 mM
PH_METHOD buffer

Order Parameters PAPC

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