Experiment OP - 10.1021/bi00595a029/1

Article DOI 10.1021/bi00595a029
Data DOI N/A
Internal ID 10.1021/bi00595a029/1
Membrane composition (molar fraction)
DPPC 1,2-dipalmitoyl-sn-glycero-3-phosphocholine 1
Solution composition
Compound Mass %
ACE 0.59
SOD 0.23
Temperature (K) 331
Total hydration 50 (mass %)
pH 5.7
Reagent sources
DPPC Synthesized locally, specifically deuterated at the choline alpha (+NCD2CH2-DPPC), beta (+NCH2CD2-DPPC), and gamma ((CD3)3N+-DPPC) segments
water Unknown
EDTA Unknown
acetic_acid Unknown
sodium_acetate Unknown
Sample protocol Appropriate amounts of lipid were dissolved in chloroform/methanol. Solvent was removed under nitrogen followed by at least 24 h under high vacuum. Buffer was added to yield a final lipid:buffer ratio of 50:50 wt %. Ampoules were sealed and mixed above the phase transition temperature of pure phospholipids.
NMR method 2H:SP
NMR instrument Bruker-Spectrospin HX-90-FT spectrometer (13.8 MHz for 2H, 36.4 MHz for 31P)
NMR RF heating correction UNKNOWN
NMR sign measured NONE
NMR details The experiment investigated the influence of cholesterol on the orientation and flexibility of DPPC headgroups. Deuterium quadrupole splittings and Phosphorus-31 chemical shielding anisotropies were evaluated directly from powder-type spectra. The values reported here for pure DPPC are extracted from the 0.0 cholesterol/lipid molar ratio point in Figure 2, which corresponds to measurements at 58 °C. Direct 1H-13C NMR measurements have established that the order parameters for the alpha segment are positive, while the beta segment is negative
Show on GitHub experiments/OrderParameters/10.1021/bi00595a029/1 main

Name Value
DATE 1978
PH_METHOD buffer
ADDITIONAL_MOLECULES
Key Value
EDTA Ethylenediaminetetraacetic acid, 0.0001 M
Acetic_acid sodium acetate-acetic acid, 0.2 M

Order Parameters DPPC

View JSON main