Experiment OP - 10.1021/bi00231a015/1

Article DOI 10.1021/bi00231a015
Data DOI N/A
Internal ID 10.1021/bi00231a015/1
Membrane composition (molar fraction)
PAPC 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine 1
Solution composition Pure water
Temperature (K) 303
Total hydration 50 (mass %)
pH 7.4
Reagent sources
PAPC (16:0)(20:4-d8)PC was synthesized in the study
H2O Deuterium-depleted water
Sample protocol The sample was subjected to freeze-thaw-vortex cycles under Ar(g) to ensure complete dispersion of the lipid; it was vortexed less than a minute each time to minimize the formation of small liposomes or vesicles.
NMR method 2H:QE
NMR instrument GN-300 (GE, Vermont) at 46.13 MHz equipped with a home-built high-power transverse solenoid probe.
NMR RF heating correction UNKNOWN
NMR sign measured NONE
NMR details The 90° pulse length was typically 6 µs. A phase-cycled quadrupolar echo pulse sequence (Davis et al., 1976) was employed with a pulse spacing of 40 µs. Data were acquired beginning at 30 µs after the second pulse and were Fourier transformed carefully beginning at the top of the echo. The pulse sequence was repeated every 500 ms, and the spectra were acquired with a digitization rate (spectral width) of 500 kHz.
Show on GitHub experiments/OrderParameters/10.1021/bi00231a015/1 main

Name Value
ADDITIONAL_MOLECULES
Key Value
TRIS tris(hydroxymethyl)aminomethane, 20 mM
EDTA Ethylenediaminetetraacetic acid, 0.1 mM
PH_METHOD buffer

Order Parameters PAPC

View JSON main