Experiment OP - 10.1021/bi00218a003/1

Article DOI 10.1021/bi00218a003
Data DOI N/A
Internal ID 10.1021/bi00218a003/1
Membrane composition (molar fraction)
PILPC 1-Palmitoyl-2-isolinoleoyl phosphatidylcholine 1
Solution composition Pure water
Temperature (K) 313
Total hydration 85 (mass %)
pH UNKNOWN
Reagent sources
PILPC Deuterated isolinoleoyl phosphatidylcholine lipids synthesized as described in the original publication. Lipids were purified using thin-layer chromatography and column chromatography.
H2O Deuterium-depleted water
Sample protocol Lipids were dissolved in chloroform, dried under argon, and subsequently lyophilized. Samples were hydrated with deuterium-depleted water (~200 µL for ~35 mg lipid), followed by freeze–thaw–vortex cycles to form multilamellar vesicles.
NMR method 2H:QE
NMR instrument Bruker MSL-300 (46.06 MHz) and custom-built spectrometer (30.7 MHz)
NMR RF heating correction UNKNOWN
NMR sign measured NONE
NMR details 2H NMR quadrupolar echo experiments were performed to obtain spectra. Spectra were dePaked to extract segmental order parameters.
Show on GitHub experiments/OrderParameters/10.1021/bi00218a003/1 main

Name Value
ADDITIONAL_MOLECULES
Key Value
PH_METHOD UNKNOWN

Order Parameters PILPC

View JSON main