Experiment OP - 10.1016/S0006349594809348/1

Article DOI 10.1016/S0006-3495(94)80934-8
Data DOI N/A
Internal ID 10.1016/S0006349594809348/1
Membrane composition (molar fraction)
DOPC 1,2-dioleoyl-sn-glycero-3-phosphocholine 1
Solution composition Pure water
Temperature (K) 303
Total hydration 33.5 (mass %)
pH UNKNOWN
Reagent sources
DOPC Synthetic dioleoylphosphatidylcholine (DOPC), specifically deuterated at the alpha, beta, and gamma segments of the choline headgroup (DOPC-d4 and DOPC-d9)
water Deuterium-depleted water (Aldrich)
Sample protocol Lipid dissolved in chloroform was dried under a stream of nitrogen and high vacuum overnight. Multilamellar liposomes were prepared by adding a well-defined amount of deuterium-depleted water using a microsyringe. Samples were homogenized and equilibrated at 30°C
NMR method 2H:SP
NMR instrument Homebuilt spectrometer operating at a deuterium frequency of 55.3 MHz
NMR RF heating correction UNKNOWN
NMR sign measured NONE
NMR details Quadrupole splittings were recorded over a range of water/lipid mole ratios from 4 to 100. The values reported for the databank are the limiting plateau values (f_s from Table 1) representing the fully hydrated state at 303 K. Signs determined as described in http://arxiv.org/abs/1309.2131v2.
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Name Value
DATE 1994

Order Parameters DOPC

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